如何使用SHI-FIX對(duì)懸浮細(xì)胞做免疫熒光？

您是否希望懸浮細(xì)胞的免疫熒光像貼壁細(xì)胞一樣容易？現(xiàn)在是這樣！Shi-fix™（由Shikhar生物技術(shù)公司制造）蓋滑允許懸浮細(xì)胞分層或直接作為單層生長。簡單，無憂的實(shí)驗(yàn)方案，無需離心柱即可將懸浮細(xì)胞固定到載玻片上。只需將細(xì)胞添加到蓋玻片中，等待30分鐘，用PBS洗滌未結(jié)合的細(xì)胞并繼續(xù)免疫染色（或繼續(xù)培養(yǎng)以獲得所需的細(xì)胞密度）。

這些蓋玻片也可用于染色懸浮細(xì)胞以進(jìn)行顯微鏡檢查，或用于固定懸浮細(xì)胞以進(jìn)行共聚焦顯微鏡檢查。更重要的是，如果需要，您還可以在細(xì)胞附著在蓋玻片上時(shí)刺激它們。您可以像貼壁細(xì)胞一樣處理非貼壁細(xì)胞！

Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).

These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!

SHI-FIX cover-slips玻片做的懸浮細(xì)胞免疫熒光實(shí)驗(yàn)圖：

SHI-FIX cover-slips玻片的操作步驟：簡單！方便！！

SHI-FIX cover-slips玻片的產(chǎn)品訂購信息：